Microbial genetics rna and protein synthesis, the genetic code lecture 12 handout. The key property of transposons is that a copy of the entire transposon sequence can at a low frequency become inserted at a new chromosomal site. A library of over 2,000 free lectures we are on a mission to create a library of lectures that provide simple explanations to many difficult topics in the sciences. Microbial genetics types of mutations, mutagenic agents, identifying mutants and mutagens lecture 14 handout. Powerpoint is the worlds most popular presentation software which can let you create professional bacterial genetics powerpoint presentation easily and in no time. The ways bacteria regulate enzyme activity exotoxins and endotoxins, transmission, adhesion, immunopathology. The key property of transposons is that a copy of the entire transposon sequence can at a low frequency become inserted at a. The microbial world introduction to the procaryotic cell reading assignment. The molecular mechanisms involved in catabolite repression and attenuation. Microbiology 2421 lecture notes microbial genetics and.
Lecture 1 we will begin this course with the question. If you have watched this lecture and know what it is about, particularly what biology topics are discussed, please help us by commenting on this video with your suggested description and title. Transposons are usually from 103 to 104 base pairs in length, depending on the transposon type. Free download bacterial genetics powerpoint presentation. Find materials for this course in the pages linked along the left. Microbial genetics lecture materials from the virtual. Many spo powerpoints are available in a variety of formats, such as fully editable powerpoint files. The physical definition of the gene is a very good one but there are many instances where we wish to study genes whose dna sequences are not known. Biochemists, for the most part, study how proteins work. Characteristics a much smaller than the chromosome pdf files introduction to microbiology. Bacterial genetics, lectures 3 st replication dna regulation change mutation.
Notes molecular genetics, lectures 916 20 pages notes molecular genetics, lectures 18 10 pages lecture support, lecture 26 consists out of objectives, key terms and revision questions molecular genetics lectures 1 and 2 molecular biology lecture notes 121 revision questions for lecture 212 molec bio. To get purer dna from either 1 or 2, crude dna is a fractionated on a cscl 2 gradient b precipitated with ethanol c poured over a resin column that specifically binds dna b. That uses up less paper, placing two slides on each page. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Structure and function of the genetic material, dna replication, the genetic code, gene structure, the expression and regulation of genes, mutations and their chemical basis, detection and isolation of mutants, dna repair, microbial recombination and bacterial plasmids, transposable elements, bacterial. Bacterial structure characteristic gramnegative bacteria grampositive bacteria wall structure they have a thin lipopolysaccharide exterior cell wall. Learn vocabulary, terms, and more with flashcards, games, and other study tools.
Fundamental bacterial genetics presents a concise introduction to microbial genetics. And what genetics does, steadying function, its enable one to discover genes. Circular chromosomes prokaryotic dna is composed of doublestranded antiparallel dna strand. Classification of chromosomes based on position of centromere. Compare and contrast vertical and horizontal gene transfer. Microbial genetics genetic transfer and recombination in bacteria lecture 15 handout. Regulation of bacterial gene expression constitutive. Download englishus transcript pdf so in this last lecture, what id like to do is id like to now begin to talk about genetics. Describe the essential nutrients required for bacterial growth 4. Freely browse and use ocw materials at your own pace. Bacterial conjugation hfr, f prime and f plasmid duration. Microbial physiology is a 3 credit course with two 1 hour 20 minute lectures each week. Explain the phenomenon of transposition and its role introducing genetic variability in prokaryotic genomes.
Please give the authors the credit with proper references. An agentbased approach to correctness in databases. And when we talked early on in the semester i was showing you this way that you can study biological function. Microbial physiology is an intensive course with the goal of integrating biochemistry and. Microbial genetics regulation by repression, induction and attenuation lecture handout. Microbial genetics jaya seelan sathiya seelan phd institute for tropical biology and conservation, ums 2. Knowledge on dna lead to advances in molecular biology central dogma of life deoxyribonucleic acid dna carries the genetic information dna is transcribed to rna polypeptides cell function depends upon specific polypeptides proteins enzymes dna is a store house of. Start studying lecture 4 bacterial genetics and molecular biology. When new viruses are formed in one host bacterial cell, the bacterias dna can accidentally be enclosed in a viral protein coat rather than the viral dna.
From the germ theory of disease to the genomics era module 1 the bacterial cell structure of the bacterial cell. The peptidoglycan layer is thick effect of dye do not retain the crystal violet dye, and react only with a counterstain, generally stain pink. Principles of bacterial sex 251 elements of yeast genetics 253. A solid balance of concepts, techniques and applications makes this book an accessible, essential introduction to the.
Knowledge on dna lead to advances in molecular biology. For example, say we have isolated a new mutant fly that is also paralyzed and we want to know whether this mutation is also in the shibire gene. Microbiology 2421 lecture notes microbial genetics and biotechnology dr. Antibiotic resistance is one phenotype of genetic transfer between bacteria and that the same principles allow other genes like pathogenicity and virulence factors to spread. Bacterial artificial chromosomes bacs played an important role in the human genome project can see in the picture that there are an number of genetic elements needed, the lacz gene is present in order to conduct the blue and white screen to screen for recombinants and there is also an origin of replication and genes for the replication. Pdf short course in bacterial genetics researchgate. Levinson, chapter 1, 2 omit plasmids and transposons until genetics lectures, and 5 classes of microorganisms which classes contain human pathogens. Mar 04, 2010 the structure and transcription of bacterial genes. Haploidy possessing only one set of genes allows the expression of mutations immediately. This question will take us four lectures to answer because there are actually several different definitions that are appropriate in different contexts. Conjugation, transformation and transduction youtube. The genome is supercoiled with one negative turn ever 200 bptwists, which aids in compaction of the dna inside the cell. Bacterial conjugation requires an f factor plasmid has all conjugation genes directs formation of a sex pilus single dna strand produced by dna replication is transferred to f cell through the sex pilus, recipient produces 2nd strand. Dna can be cut into large fragments by mechanical shearing.
It is not surprising that the development of genetic analysis in microorganisms should have lagged for almost 40 years. Introduction to microbial genetics questions and study. Development of genetics and molecular biology 1866 genetics start to get attention when mendel experimented with green peas and publish his finding 1910 morgan revealed that the units of heredity are contained with chromosome, 1944 it is confirmed through studies on the bacteria that it was dna that carried the genetic information. This video lecture, part of the series principles of microbiology with heidi bulfer by prof. This helps you give your presentation on bacterial genetics in a conference, a school lecture, a business proposal, in a webinar and business and professional representations the uploader spent. Molecular biology lecture notes, lectures 1 22, complete. The text focuses on one bacterial species, escherichia coli, but draws examples from other microbial systems at appropriate points to support the fundamental concepts of molecular genetics. Genetic exchange mediated by bacterial viruses bacteriophage two basic types of bacterial viruses lytic viruses infect cells, multiply rapidly, lyse cells lysogenic viruses infect cells, can integrate into genome and go dormant a prophage. Modern microbial genetics, second edition wiley online. Learn lecture notes microbial genetics with free interactive flashcards. Levinson, chapter 1, 2 omit plasmids and transposons until genetics lectures, and 5 classes of microorganisms. Lecture 12 transposable elements mit opencourseware. Bacterial enumeration dilutions and plate counts and submitted the lab assessment pdf to blackboard chapter 8 bacterial genetics viewed the lectures and took notes read the textbook completed the chapter 8. In recombination, a new chromosome with a genotype different from that of the parent results from the combination of genetic material from.
Choose from 500 different sets of lecture notes microbial genetics flashcards on quizlet. We think that every difficult topic can be explained in simple terms and it is our aim to do just that in the shortest amount of time. Once the replicated plasmid and bacterial chromosome reach the recipient cell there is a greater chance of integration. Select print, and, when the print screen comes up, go to the print handling options.
This rarely happens due to spontaneous break down of the replicated dna or f pilus. We are on a mission to create a library of lectures that provide simple explanations to many difficult topics in the sciences. The lectures often summarize material of a chapter and then discuss in depth a recent paper that extends the material of the chapter. Microbial genetics introduction u genetics is the science of heredity. Describe the mechanisms of genetic variation in bacterial cell 5. In recombination, a new chromosome with a genotype different from that of the parent results from the combination of genetic material from two organisms.
The molecular mechanisms that bacteria use to regulate gene activity. In accordance with its predecessor, the completely revised and expanded second edition of modern microbial genetics focuses on how bacteria and bacteriophage arrange and rearrange their genetic material through mutation, evolution, and genetic exchange to take optimal advantage of their environment. Lecture 4 bacterial genetics and molecular biology. Lecture 12 transposable elements transposons are usually from 103 to 104 base pairs in length, depending on the transposon type. To understand the role of genetics in defining biological phenomena 2. Pdf files corresponding to these papers are available on a course. The course is taught by one instructor whom is faculty in the department of biochemistry and microbiology.
Semmelweis and lister from the amazing blood and guts series. Segment of dna or rna in some viruses that codes for functional products. It can be found in soil, air, water, and living bodies. The best way for students to print out the powerpoint show is to download the pdf version. Promotor site where rna polymerase binds necessary for transcription b. Some bacteria can cause diseases for human, animals and plants. Bacterial morphology and structure bacterial physiology disinfection and sterilization bacteriophage bacterial variation bacterial infection and immunity laboratory diagnosis, prevention and treatment of bacte rial infection the staphylococci enteric bacilli vibrio anaerobic bacteria actinomyces and nocardia diphtheria mycobacterium brucella. The transfer of the replicated plasmid and bacterial chromosome takes approximately 90 minutes. Lecture 1 intro to genetics 20% genetic disease classic medical genetics, single gene, early onset pediatric 80% genetic susceptibility common gene variation and environment, delayed onset adult pedigree children, siblings, parents nuclear family agedate birth, health status, agedate death, cause of death. Under page scaling, select multiple pages per sheet, under pages per sheet, select 2. Operon genes segments of dna on bacterial chromosome regulate this process 1. Small, circular dna molecules that can exist independently of the chromosome 2. Identify the chemical meanses of sterilization and disinfection, and their effect on bacterial cell 6. Microbial genetics lecture powerpoint science prof online.
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